| Host: | Mouse |
| Applications: | WB/IHC/FC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-HGS for use in WB, IHC and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | 6C2E1 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. NA |
| Purification: | Lyophilized |
| Concentration: | Adding 0.2 mL of distilled water =f 500 Mu g/mL. |
| Storage Instruction: | Store at-20°C for up to 1 year. Minimise freeze-thaw cycles. |
| Gene Symbol: | HGS |
| Gene ID: | 9146 |
| Uniprot ID: | HGS_HUMAN |
| Immunogen: | E.coli-derived human HGS recombinant protein (Position: R3-D777). |
| Post Translational Modifications | Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected. Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF. Ubiquitinated. Ubiquitinated by ITCH. |
| Function | Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. |
| Protein Name | Hepatocyte Growth Factor-Regulated Tyrosine Kinase SubstrateHrsProtein Pp110 |
| Database Links | Reactome: R-HSA-182971Reactome: R-HSA-432720Reactome: R-HSA-5689880Reactome: R-HSA-6807004Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8875360Reactome: R-HSA-9013420Reactome: R-HSA-917729Reactome: R-HSA-9635644Reactome: R-HSA-9636383Reactome: R-HSA-9706019 |
| Cellular Localisation | CytoplasmEarly Endosome MembranePeripheral Membrane ProteinCytoplasmic SideEndosomeMultivesicular Body MembraneColocalizes With Ubqln1 In Ubiquitin-Rich Cytoplasmic Aggregates That Are Not Endocytic Compartments |
| Alternative Antibody Names | Anti-Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate antibodyAnti-Hrs antibodyAnti-Protein Pp110 antibodyAnti-HGS antibodyAnti-HRS antibody |
Information sourced from Uniprot.org
