• Western blot analysis of extracts of various cell lines, using HDGFRP3 antibody (STJ111429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using HDGFRP3 Rabbit polyclonal antibody (STJ111429) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat spinal cord using HDGFRP3 antibody (STJ111429) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using HDGFRP3 Rabbit polyclonal antibody (STJ111429) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human uterine cancer using HDGFRP3 antibody (STJ111429) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using HDGFRP3 antibody (STJ111429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDGFRP3 antibody (90-203) (STJ111429)

SKU:
STJ111429

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HDGFRP3 (90-203) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDGFL3
Gene ID: 50810
Uniprot ID: HDGR3_HUMAN
Immunogen Region: 90-203
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-203 of human HDGFRP3 (NP_057157.1).
Immunogen Sequence: NNPGVKFTGYQAIQQQSSSE TEGEGGNTADASSEEEGDRV EEDGKGKRKNEKAGSKRKKS YTSKKSSKQSRKSPGDEDDK DCKEEENKSSSEGGDAGNDT RNTTSDLQKTSEGT
Function Enhances DNA synthesis and may play a role in cell proliferation.
Protein Name Hepatoma-Derived Growth Factor-Related Protein 3
Hrp-3
Hepatoma-Derived Growth Factor 2
Hdgf-2
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatoma-Derived Growth Factor-Related Protein 3 antibody
Anti-Hrp-3 antibody
Anti-Hepatoma-Derived Growth Factor 2 antibody
Anti-Hdgf-2 antibody
Anti-HDGFL3 antibody
Anti-HDGF2 antibody
Anti-HDGFRP3 antibody
Anti-CGI-142 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance