• Western blot analysis of lysates from NIH/3T3, HeLa, and Jurkat cells,, using HDAC8 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293-UV cells using HDAC8 Polyclonal Antibody diluted at 1：1000
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HDAC8 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using HDAC8 Polyclonal Antibody diluted at 1：1000

Anti-HDAC8 antibody (5-54 aa) (STJ93485)

SKU:
STJ93485

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histone deacetylase 8 (5-54 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC8
Gene ID: 55869
Uniprot ID: HDAC8_HUMAN
Immunogen Region: 5-54 aa
Specificity: HDAC8 Polyclonal Antibody detects endogenous levels of HDAC8 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HDAC8 at the amino acid range 5-54
Post Translational Modifications Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
Function Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones.
Protein Name Histone Deacetylase 8
Hd8
Protein Deacetylase Hdac8
Protein Decrotonylase Hdac8
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2467813 Q9BY41-1
Reactome: R-HSA-2500257 Q9BY41-1
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3214815
Reactome: R-HSA-350054
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Excluded From The Nucleoli
Found In The Cytoplasm Of Cells Showing Smooth Muscle Differentiation
Alternative Antibody Names Anti-Histone Deacetylase 8 antibody
Anti-Hd8 antibody
Anti-Protein Deacetylase Hdac8 antibody
Anti-Protein Decrotonylase Hdac8 antibody
Anti-HDAC8 antibody
Anti-HDACL1 antibody
Anti-CDA07 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance