| Host: |
Rabbit |
| Applications: |
WB/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-HDAC3 (329-428) is suitable for use in Western Blot and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HDAC3 |
| Gene ID: |
8841 |
| Uniprot ID: |
HDAC3_HUMAN |
| Immunogen Region: |
329-428 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 329-428 of human HDAC3 (NP_003874.2). |
| Immunogen Sequence: |
FEYFAPDFTLHPDVSTRIEN QNSRQYLDQIRQTIFENLKM LNHAPSVQIHDVPADLLTYD RTDEADAEERGPEENYSRPE APNEFYDGDHDNDKESDVEI |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Sumoylated in vitro. Deubiquitinated on 'Lys-63'-linked ubiquitin chains by USP38.leading to a decreased level of histone acetylation. |
| Function | Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress. Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner. During the activation phase, promotes the accumulation of ubiquitinated BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and BMAL1. The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene BMAL1 and the genes involved in lipid metabolism in the liver. Also functions as deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14 and RARA. Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response. In addition to protein deacetylase activity, also acts as protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation and de-2-hydroxyisobutyrylation, respectively. Catalyzes decrotonylation of MAPRE1/EB1. |
| Protein Name | Histone Deacetylase 3Hd3Protein Deacetylase Hdac3Protein Deacylase Hdac3Rpd3-2Smap45 |
| Database Links | Reactome: R-HSA-1368071Reactome: R-HSA-193670Reactome: R-HSA-1989781Reactome: R-HSA-2122947Reactome: R-HSA-2151201Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-3214815Reactome: R-HSA-350054Reactome: R-HSA-381340Reactome: R-HSA-390471Reactome: R-HSA-400206Reactome: R-HSA-400253Reactome: R-HSA-5617472Reactome: R-HSA-8940973Reactome: R-HSA-8943724Reactome: R-HSA-9022537Reactome: R-HSA-9022692Reactome: R-HSA-9029569Reactome: R-HSA-9609690Reactome: R-HSA-9701898Reactome: R-HSA-9707564Reactome: R-HSA-9707616 |
| Cellular Localisation | NucleusCytoplasmCytosolColocalizes With Xbp1 And Akt1 In The CytoplasmPredominantly Expressed In The Nucleus In The Presence Of Ccar2 |
| Alternative Antibody Names | Anti-Histone Deacetylase 3 antibodyAnti-Hd3 antibodyAnti-Protein Deacetylase Hdac3 antibodyAnti-Protein Deacylase Hdac3 antibodyAnti-Rpd3-2 antibodyAnti-Smap45 antibodyAnti-HDAC3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
