| Host: | Mouse |
| Applications: | WB/ICC/IF |
| Reactivity: | Human/Mouse/Rat/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-HDAC2 for use in WB, ICC and IF in Human, Mouse, Rat and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | 2D9-F6-G7 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Formulation: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Purification: | Affinity Purified |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:1000IF 1:50-1:200 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | HDAC2 |
| Gene ID: | 3066 |
| Uniprot ID: | HDAC2_HUMAN |
| Immunogen: | Purified recombinant human HDAC2 protein fragments expressed in E.coli. |
| Tissue Specificity | Widely expressed.lower levels in brain and lung. |
| Post Translational Modifications | S-nitrosylated by GAPDH. In neurons, S-nitrosylation at Cys-262 and Cys-274 does not affect enzyme activity, but induces HDAC2 release from chromatin. This in turn increases acetylation of histones surrounding neurotrophin-dependent gene promoters and promotes their transcription. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. |
| Function | Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Component of the SIN3B complex that represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2. Also deacetylates non-histone targets: deacetylates TSHZ3, thereby regulating its transcriptional repressor activity. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively. |
| Protein Name | Histone Deacetylase 2Hd2Protein Deacylase Hdac2 |
| Database Links | Reactome: R-HSA-193670Reactome: R-HSA-2122947Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-3214815Reactome: R-HSA-350054Reactome: R-HSA-427389Reactome: R-HSA-427413Reactome: R-HSA-4551638Reactome: R-HSA-6804758Reactome: R-HSA-73762Reactome: R-HSA-8943724Reactome: R-HSA-9022692Reactome: R-HSA-9022699Reactome: R-HSA-9615017Reactome: R-HSA-9619665Reactome: R-HSA-9679191Reactome: R-HSA-9701898Reactome: R-HSA-983231Reactome: R-HSA-9843940Reactome: R-HSA-9844594Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Histone Deacetylase 2 antibodyAnti-Hd2 antibodyAnti-Protein Deacylase Hdac2 antibodyAnti-HDAC2 antibody |
Information sourced from Uniprot.org