• Immunofluorescence analysis of paraffin-embedded mouse brain using HCRTR1 Rabbit polyclonal antibody (STJ116940) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from C6 cells, using HCRTR1 Rabbit polyclonal antibody (STJ116940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HCRTR1 antibody (150-250) (STJ116940)

SKU:
STJ116940

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCRTR1 (150-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HCRTR1
Gene ID: 3061
Uniprot ID: OX1R_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2).
Immunogen Sequence: HPLLFKSTARRARGSILGIW AVSLAIMVPQAAVMECSSVL PELANRTRLFSVCDERWADD LYPKIYHSCFFIVTYLAPLG LMAMAYFQIFRKLWGRQIPG T
Function Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.
Protein Name Orexin/Hypocretin Receptor Type 1
Hypocretin Receptor Type 1
Orexin Receptor Type 1
Ox-1-R
Ox1-R
Ox1r
Database Links Reactome: R-HSA-389397
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Orexin/Hypocretin Receptor Type 1 antibody
Anti-Hypocretin Receptor Type 1 antibody
Anti-Orexin Receptor Type 1 antibody
Anti-Ox-1-R antibody
Anti-Ox1-R antibody
Anti-Ox1r antibody
Anti-HCRTR1 antibody

Information sourced from Uniprot.org

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