• Western blot analysis of lysates from Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2 ECDHis Tag) , using HCoV-HKU1 Spike S1 Rabbit polyclonal antibody (STJ11103404) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HCoV-HKU1 Spike S1 antibody (100-200) (STJ11103404)

SKU:
STJ11103404

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Host: Rabbit
Applications: WB/ELISA
Reactivity: HCoV-HKU1
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of coronavirus Spike S1 (YP_173238.1).
Immunogen Sequence: NGIFSRVKNTKLYVNKTLYS EFSTIVIGSVFINNSYTIVV QPHNGVLEITACQYTMCEYP HTICKSKGSSRNESWHFDKS EPLCLFKKNFTYNVSTDWLY F
Background [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance