Anti-HAND1 antibody (90-190 aa) [8E7A11/8C2F4] (STJ98125)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of HAND1 (aa90-190) expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-Heart-and neural crest derivatives-expressed protein 1 (90-190 aa) for use in WB and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 8E7A11/8C2F4 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HAND1 |
| Gene ID | 9421 |
| Uniprot ID | HAND1_HUMAN |
| Immunogen | Purified recombinant fragment of HAND1 (aa90-190) expressed in E. Coli. |
| Immunogen Region | 90-190 aa |
| Specificity | HAND1 Monoclonal Antibody detects endogenous levels of HAND1 protein. |
Additional Info
| Post Translational Modifications | Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to translocation into the nucleoplasm, allowing dimerization and transcription factor activity. |
| Function | Transcription factor that plays an essential role in both trophoblast giant cell differentiation and in cardiac morphogenesis. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box). Acts as a transcriptional repressor of SOX15. In the adult, could be required for ongoing expression of cardiac-specific genes. |
| Protein Name | Heart- And Neural Crest Derivatives-Expressed Protein 1Class A Basic Helix-Loop-Helix Protein 27Bhlha27Extraembryonic Tissues - Heart - Autonomic Nervous System And Neural Crest Derivatives-Expressed Protein 1Ehand |
| Database Links | Reactome: R-HSA-9733709 |
| Cellular Localisation | NucleusNucleoplasmNucleolusInteraction With Mdfic Sequesters It Into The NucleolusPreventing The Transcription Factor ActivityPhosphorylation By Plk4 Disrupts The Interaction With Mdfic And Releases It From The NucleolusLeading To Transcription Factor Activity |
| Alternative Antibody Names | Anti-Heart- And Neural Crest Derivatives-Expressed Protein 1 antibodyAnti-Class A Basic Helix-Loop-Helix Protein 27 antibodyAnti-Bhlha27 antibodyAnti-Extraembryonic Tissues - Heart - Autonomic Nervous System And Neural Crest Derivatives-Expressed Protein 1 antibodyAnti-Ehand antibodyAnti-HAND1 antibodyAnti-BHLHA27 antibodyAnti-EHAND antibody |
Information sourced from Uniprot.org