Anti-H3-3A/H3-3B antibody (16-65 aa) (STJ93535)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Histone H3.3 at the amino acid range 16-65
General Information
| Short Description | Rabbit polyclonal anti-Histone H3.3 (16-65 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | H3-3A.H3-3B |
| Gene ID | 3020/3021 |
| Uniprot ID | H33_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Histone H3.3 at the amino acid range 16-65 |
| Immunogen Region | 16-65 aa |
| Specificity | Histone H3.3 Polyclonal Antibody detects endogenous levels of Histone H3.3 protein. |
Additional Info
| Function | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Protein Name | Histone H3.3 |
| Database Links | Reactome: R-HSA-1912408Reactome: R-HSA-201722Reactome: R-HSA-212300Reactome: R-HSA-2299718Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-427359Reactome: R-HSA-427389Reactome: R-HSA-427413Reactome: R-HSA-5250924Reactome: R-HSA-5334118Reactome: R-HSA-5578749Reactome: R-HSA-5617472Reactome: R-HSA-5625886Reactome: R-HSA-68616Reactome: R-HSA-73728Reactome: R-HSA-73772Reactome: R-HSA-8936459Reactome: R-HSA-8939236Reactome: R-HSA-9018519Reactome: R-HSA-912446Reactome: R-HSA-9616222Reactome: R-HSA-9670095Reactome: R-HSA-9710421Reactome: R-HSA-977225Reactome: R-HSA-9821002Reactome: R-HSA-9821993Reactome: R-HSA-983231Reactome: R-HSA-9841922Reactome: R-HSA-9843940Reactome: R-HSA-9843970Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Histone H3.3 antibodyAnti-H3-3A antibodyAnti-H3.3A antibodyAnti-H3F3 antibodyAnti-H3F3A antibodyAnti-PP781.H3-3B antibodyAnti-H3.3B antibodyAnti-H3F3B antibody |
Information sourced from Uniprot.org