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Anti-H3-3A/H3-3B antibody (16-65 aa) (STJ93535)

Immunohistochemistry analysis of paraffin-embedded human brain, using Histone H3.3 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Histone H3.3 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of Hela cells using Histone H3.3 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunofluorescence analysis of Hela cell. 1, Histone H3.3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-H3-3A/H3-3B antibody (16-65 aa) (STJ93535)

SKU:

STJ93535

£46.00 - £226.00

Current Stock:

General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Histone H3.3 (16-65 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:5000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	H3-3A.H3-3B
Gene ID:	3020/3021
Uniprot ID:	H33_HUMAN
Immunogen Region:	16-65 aa
Specificity:	Histone H3.3 Polyclonal Antibody detects endogenous levels of Histone H3.3 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Histone H3.3 at the amino acid range 16-65

Function	Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name	Histone H3.3
Database Links	Reactome: R-HSA-1912408 Reactome: R-HSA-201722 Reactome: R-HSA-212300 Reactome: R-HSA-2299718 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-427359 Reactome: R-HSA-427389 Reactome: R-HSA-427413 Reactome: R-HSA-5250924 Reactome: R-HSA-5334118 Reactome: R-HSA-5578749 Reactome: R-HSA-5617472 Reactome: R-HSA-5625886 Reactome: R-HSA-68616 Reactome: R-HSA-73728 Reactome: R-HSA-73772 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-9018519 Reactome: R-HSA-912446 Reactome: R-HSA-9616222 Reactome: R-HSA-9670095 Reactome: R-HSA-9710421 Reactome: R-HSA-977225 Reactome: R-HSA-983231
Cellular Localisation	Nucleus Chromosome
Alternative Antibody Names	Anti-Histone H3.3 antibody Anti-H3-3A antibody Anti-H3.3A antibody Anti-H3F3 antibody Anti-H3F3A antibody Anti-PP781.H3-3B antibody Anti-H3.3B antibody Anti-H3F3B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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