| Short Description : | Rat monoclonal anti-H-2 for use in Block, FC, IF and IP in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | Block/FC/IF/IP |
| Host: | Rat |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | M1/42.3.9.8 |
| Isotype: | IgG2ak |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Formulation: | 0.2 µm filtered in PBS, pH7.2. Azide Free (AF) /Low Endotoxin (LE) : Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as determined by LAL gel clotting assay. |
| Storage Instruction: | This product can be stored at 2-8°C for 12 months. Do not freeze. |
| Background | The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. |
Information sourced from Uniprot.org
![C57BL/6 murine splenocytes were stained with Anti-H-2 antibody (STJA0001066) [Used at 0.2 µg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/145919/292576/STJA0001066_1__19090.1631206452.jpg?c=1 "C57BL/6 murine splenocytes were stained with Anti-H-2 antibody (STJA0001066) [Used at 0.2 µg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.")