• Western blot analysis of extracts of various cell lines, using GTF3C4 antibody (STJ111641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using GTF3C4 Polyclonal Antibody (STJ111641) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GTF3C4 Polyclonal Antibody (STJ111641) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GTF3C4 Polyclonal Antibody (STJ111641) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using GTF3C4 Polyclonal Antibody (STJ111641) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GTF3C4 antibody (STJ111641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GTF3C4 antibody (583-822) (STJ111641)

SKU:
STJ111641

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GTF3C4 (583-822) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTF3C4
Gene ID: 9329
Uniprot ID: TF3C4_HUMAN
Immunogen Region: 583-822
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 583-822 of human GTF3C4 (NP_036336.2).
Immunogen Sequence: ILYQSMQKTPSEALWKPTHE DSKILLVDSPGMGNADDEQQ EEGTSSKQVVKQGLQERSKE GDVEEPTDDSLPTTGDAGGR EPMEEKLLEIQGKIEAVEMH LTREHMKRVLGEVYLHTWIT ENTSIPTRGLCNFLMSDEEY DDRTARVLIGHISKKMNKQT FPEHCSLCKEILPFTDRKQA VCSNGHIWLRCFLTYQSCQS LIYRRCLLHDSIARHPAPED PDWIKRLLQSPCPFCDSPV
Function Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
Protein Name General Transcription Factor 3c Polypeptide 4
Tf3c-Delta
Transcription Factor Iiic 90 Kda Subunit
Tfiiic 90 Kda Subunit
Tfiiic90
Transcription Factor Iiic Subunit Delta
Database Links Reactome: R-HSA-749476
Reactome: R-HSA-76061
Reactome: R-HSA-76066
Cellular Localisation Nucleus
Alternative Antibody Names Anti-General Transcription Factor 3c Polypeptide 4 antibody
Anti-Tf3c-Delta antibody
Anti-Transcription Factor Iiic 90 Kda Subunit antibody
Anti-Tfiiic 90 Kda Subunit antibody
Anti-Tfiiic90 antibody
Anti-Transcription Factor Iiic Subunit Delta antibody
Anti-GTF3C4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance