• Immunofluorescence analysis of L929 cells using GST3/GST3/GSTP1 Rabbit polyclonal antibody (STJ27658) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using GST3/GST3/GSTP1 antibody (STJ27658) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using GST3/GST3/GSTP1 antibody (STJ27658) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GST3/GST3/GSTP1 antibody (STJ27658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GSTP1 antibody (1-210) (STJ27658)

SKU:
STJ27658

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GSTP1 (1-210) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSTP1
Gene ID: 2950
Uniprot ID: GSTP1_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human GST3/GST3/GSTP1 (NP_000843.1).
Immunogen Sequence: MPPYTVVYFPVRGRCAALRM LLADQGQSWKEEVVTVETWQ EGSLKASCLYGQLPKFQDGD LTLYQSNTILRHLGRTLGLY GKDQQEAALVDMVNDGVEDL RCKYISLIYTNYEAGKDDYV KALPGQLKPFETLLSQNQGG KTFIVGDQISFADYNLLDLL LIHEVLAPGCLDAFPLLSAY VGRLSARPKLKAFLASPEYV NLPINGNGKQ
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). Participates in the formation of novel hepoxilin regioisomers. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Protein Name Glutathione S-Transferase P
Gst Class-Pi
Gstp1-1
Database Links Reactome: R-HSA-156590
Reactome: R-HSA-3299685
Reactome: R-HSA-6798695
Reactome: R-HSA-9753281
Cellular Localisation Cytoplasm
Mitochondrion
Nucleus
The 83 N-Terminal Amino Acids Function As Un Uncleaved Transit Peptide
And Arginine Residues Within It Are Crucial For Mitochondrial Localization
Alternative Antibody Names Anti-Glutathione S-Transferase P antibody
Anti-Gst Class-Pi antibody
Anti-Gstp1-1 antibody
Anti-GSTP1 antibody
Anti-FAEES3 antibody
Anti-GST3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance