• Western blot analysis of extracts of GST protein, using GST-Tag antibody (STJ11103710) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunoprecipitation analysis of 1 Mu g extracts of GST Protein using 3 Mu g Rabbit anti GST-Tag antibody (STJ11103710). Western blot was performed from the immunoprecipitate using Rabbit anti GST-Tag antibody (STJ11103710) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 1ug extracts of GST-Tag cells using 3ug rabbit anti GST-Tag antibody (STJ11103710). Western blot was performed from the immunoprecipitate using rabbit anti GST-Tag antibody (STJ11103710) at a dilition of 1:1000.
  • Western blot analysis of lysates from GST protein, using Rabbit anti GST-Tag monoclonal antibody (STJ11103710) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-GST-Tag antibody (1-239) [S0MR] (STJ11103710)

SKU:
STJ11103710

£135.50 - £249.50
Current Stock:
Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:6000
IP:3 Mu g antibody for 1 Mu g extracts of recombinant protein
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-239
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of schja GST protein (P08515).
Immunogen Sequence: MSPILGYWKIKGLVQPTRLL LEYLEEKYEEHLYERDEGDK WRNKKFELGLEFPNLPYYID GDVKLTQSMAIIRYIADKHN MLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKV DFLSKLPEMLKMFEDRLCHK TYLNGDHVTHPDFMLYDALD VVLYMDPMCLDAFPKLVCFK KRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPK
Background Glutathione S-transferases (GSTs) , previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

Information sourced from Uniprot.org

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