• Western blot analysis of extracts of GST protein, using GST-Tag antibody (STJ11103710) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunoprecipitation analysis of 1 Mu g extracts of GST Protein using 3 Mu g Rabbit anti GST-Tag antibody (STJ11103710). Western blot was performed from the immunoprecipitate using Rabbit anti GST-Tag antibody (STJ11103710) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 1ug extracts of GST-Tag cells using 3ug rabbit anti GST-Tag antibody (STJ11103710). Western blot was performed from the immunoprecipitate using rabbit anti GST-Tag antibody (STJ11103710) at a dilition of 1:1000.
  • Western blot analysis of lysates from GST protein, using Rabbit anti GST-Tag monoclonal antibody (STJ11103710) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-GST-Tag antibody (1-239) [S0MR] (STJ11103710)

SKU:
STJ11103710

£89.50 - £248.50
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GST-Tag (1-239) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-239
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of schja GST protein (P08515).
Immunogen Sequence: MSPILGYWKIKGLVQPTRLL LEYLEEKYEEHLYERDEGDK WRNKKFELGLEFPNLPYYID GDVKLTQSMAIIRYIADKHN MLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKV DFLSKLPEMLKMFEDRLCHK TYLNGDHVTHPDFMLYDALD VVLYMDPMCLDAFPKLVCFK KRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPK
Background The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene.

Information sourced from Uniprot.org

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