• Immunofluorescence analysis of un-treated (left) A549 and UV treated (right) A549 cell. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
  • Western blot analysis of lysates from 1) Mouse-brain, 2) Hela cells treated by UV 15min, 3) HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody HRP goat anti rabbit (STJ99666) was diluted at 1:10000

Anti-GSDME antibody (1-100) (STJA0010252)

SKU:
STJA0010252

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Gasdermin-E (1-100) is suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:5000-20000
IHC 1:200-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSDME
Gene ID: 1687
Uniprot ID: GSDME_HUMAN
Immunogen Region: 1-100
Specificity: The antibody detects endogenous full lenth and n-ternal fragment of gsdme protein
Immunogen: Synthesized peptide derived from human GSDME. AA range:1-100
Post Translational Modifications Cleavage at Asp-270 by CASP3 (mature and uncleaved precursor forms) or granzyme B (GZMB) relieves autoinhibition and is sufficient to initiate pyroptosis. Gasdermin-E: Succination by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits processing by caspases, and ability to initiate pyroptosis. Succination modification is catalyzed by a non-enzymatic reaction caused by an accumulation of fumarate.
Function Gasdermin-E: Precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis. This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores, triggering pyroptosis. Gasdermin-E, N-terminal: Pore-forming protein produced by cleavage by CASP3 or granzyme B (GZMB), which converts non-inflammatory apoptosis to pyroptosis or promotes granzyme-mediated pyroptosis, respectively. After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukins (IL1B and IL16) and triggering pyroptosis. Binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. Cleavage by CASP3 switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis. Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents. Exhibits bactericidal activity. Cleavage by GZMB promotes tumor suppressor activity by triggering robust anti-tumor immunity. Suppresses tumors by mediating granzyme-mediated pyroptosis in target cells of natural killer (NK) cells: cleavage by granzyme B (GZMB), delivered to target cells from NK-cells, triggers pyroptosis of tumor cells and tumor suppression. May play a role in the p53/TP53-regulated cellular response to DNA damage. Gasdermin-E, N-terminal: (Microbial infection) Pore-forming protein, which promotes maternal placental pyroptosis in response to Zika virus infection, contributing to adverse fetal outcomes.
Protein Name Gasdermin-E
Inversely Correlated With Estrogen Receptor Expression 1
Icere-1
Non-Syndromic Hearing Impairment Protein 5 Cleaved Into - Gasdermin-E - N-Terminal
Gsdme-Nt - Gasdermin-E - C-Terminal
Gsdme-Ct
Database Links Reactome: R-HSA-111457
Reactome: R-HSA-5620971
Reactome: R-HSA-5686938
Reactome: R-HSA-9710421
Cellular Localisation Gasdermin-E
N-Terminal: Cell Membrane
Multi-Pass Membrane Protein
Gasdermin-E: Cytoplasm
Cytosol
Alternative Antibody Names Anti-Gasdermin-E antibody
Anti-Inversely Correlated With Estrogen Receptor Expression 1 antibody
Anti-Icere-1 antibody
Anti-Non-Syndromic Hearing Impairment Protein 5 Cleaved Into - Gasdermin-E - N-Terminal antibody
Anti-Gsdme-Nt - Gasdermin-E - C-Terminal antibody
Anti-Gsdme-Ct antibody
Anti-GSDME antibody
Anti-DFNA5 antibody
Anti-ICERE1 antibody

Information sourced from Uniprot.org

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