| Host: | Rabbit |
| Applications: | ICC/WB |
| Reactivity: | Bovine/Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Growth Associated Protein 43 (C-Term) for use in ICC and WB in Bovine, Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Purification: | Affinity-purified |
| Dilution Range: | WB 1:5000-1:20000ICC 1:500-1:2000 |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at-20°C to |
| Gene Symbol: | Gap43 |
| Gene ID: | 29423 |
| Uniprot ID: | NEUM_RAT |
| Immunogen Region: | C-Term |
| Specificity: | The antibody reacts with a 43 kDa band by Western blot on bovine cerebellum homogenate. The molecular weight of the protein recognized can vary (~43-57 kDa) depending on the species and the percentage acrylamide used in the SDS-PAGE gel. It has also |
| Immunogen: | C-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein. |
| Immunogen Sequence: | Rat |
| Tissue Specificity | Expressed in hippocampal neurons, with highest levels of expression in the CA4 and CA3 neurons and lower levels in CA1 neurons. Expressed in the dorsal root ganglion. |
| Post Translational Modifications | Phosphorylated. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylated by ZDHHC3. Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. |
| Function | This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. |
| Protein Name | NeuromodulinAxonal Membrane Protein Gap-43Growth-Associated Protein 43Protein F1 |
| Database Links | Reactome: R-RNO-373760 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionGrowth ConeGrowth Cone MembraneSynapseFilopodium MembranePerikaryonDendriteAxonCytoplasmCytoplasmic Surface Of Growth Cone And Synaptic Plasma Membranes |
| Alternative Antibody Names | Anti-Neuromodulin antibodyAnti-Axonal Membrane Protein Gap-43 antibodyAnti-Growth-Associated Protein 43 antibodyAnti-Protein F1 antibodyAnti-Gap43 antibody |
Information sourced from Uniprot.org