| Host: | Mouse |
| Applications: | FC/ICC/WB |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-Growth associated protein 43 (C-Term) for use in FC, ICC and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | GAP43 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Purification: | IgG |
| Dilution Range: | WB 1:5000-1:10000ICC 1:1000-1:5000 |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20°C for |
| Gene Symbol: | Gap43 |
| Gene ID: | 14432 |
| Uniprot ID: | NEUM_MOUSE |
| Immunogen Region: | C-Term |
| Specificity: | The antibody reacts with a 43 kDa band by Western blot on rat spinal cord lysate. It has also been used successfully for immunocytochemistry. |
| Immunogen: | C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein. |
| Immunogen Sequence: | Rat |
| Tissue Specificity | Expressed in the hippocampus (at protein level). Expressed in the dorsal root ganglion and the spinal cord (at protein level). |
| Post Translational Modifications | Phosphorylated. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylated by ZDHHC3. Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. |
| Function | This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. |
| Protein Name | NeuromodulinAxonal Membrane Protein Gap-43Calmodulin-Binding Protein P-57Growth-Associated Protein 43 |
| Database Links | Reactome: R-MMU-373760 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionGrowth Cone MembraneSynapseFilopodium MembranePerikaryonDendriteAxonCytoplasmCytoplasmic Surface Of Growth Cone And Synaptic Plasma Membranes |
| Alternative Antibody Names | Anti-Neuromodulin antibodyAnti-Axonal Membrane Protein Gap-43 antibodyAnti-Calmodulin-Binding Protein P-57 antibodyAnti-Growth-Associated Protein 43 antibodyAnti-Gap43 antibodyAnti-Basp2 antibody |
Information sourced from Uniprot.org
