Anti-GRK5 antibody (351-400 aa) (STJ93435)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human GRK5 at the amino acid range 351-400
General Information
| Short Description | Rabbit polyclonal anti-G protein-coupled receptor kinase 5 (351-400 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GRK5 |
| Gene ID | 2869 |
| Uniprot ID | GRK5_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human GRK5 at the amino acid range 351-400 |
| Immunogen Region | 351-400 aa |
| Specificity | GRK 5 Polyclonal Antibody detects endogenous levels of GRK 5 protein. |
Additional Info
| Post Translational Modifications | Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity. |
| Function | Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro). |
| Protein Name | G Protein-Coupled Receptor Kinase 5G Protein-Coupled Receptor Kinase Grk5 |
| Database Links | Reactome: R-HSA-416476Reactome: R-HSA-418555 |
| Cellular Localisation | CytoplasmNucleusCell MembranePeripheral Membrane ProteinPredominantly Localized At The Plasma MembraneTargeted To The Cell Surface Through The Interaction With PhospholipidsNucleus Localization Is Regulated In A Gpcr And Ca(2+)/Calmodulin-Dependent Fashion |
| Alternative Antibody Names | Anti-G Protein-Coupled Receptor Kinase 5 antibodyAnti-G Protein-Coupled Receptor Kinase Grk5 antibodyAnti-GRK5 antibodyAnti-GPRK5 antibody |
Information sourced from Uniprot.org