Anti-GPRC6A antibody (300-590) (STJ110823)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GPRC6A (300-590) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPRC6A |
| Gene ID | 222545 |
| Uniprot ID | GPC6A_HUMAN |
| Immunogen Region | 300-590 |
| Immunogen Sequence | IASDNWSTATKITTIPNVKK IGKVVGFAFRRGNISSFHSF LQNLHLLPSDSHKLLHEYAM HLSACAYVKDTDLSQCIFNH SQRTLAYKANKAIERNFVMR NDFLWDYAEPGLIHSIQLAV FALGYAIRDLCQARDCQNPN AFQPWELLGVLKNVTFTDGW NSFHFDAHGDLNTGYDVVLW KEINGHMTVTKMAEYDLQND VFIIPDQETKNEFRNLKQIQ SKCSKECSPGQMKKTTRSQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human GPRC6A (NP_683766.2). |
Additional Info
| Tissue Specificity | Isoform 1 is expressed at high level in brain, skeletal muscle, testis, bone, calvaria, osteoblasts and leukocytes. Expressed at intermediate level in liver, heart, kidney and spleen. Expressed at low level in lung, pancreas, placenta and ovary. Not detected in thymus, prostate, small intestine, tongue and colon. Isoform 1 and isoform 2 are expressed in kidney at the same level. Isoform 2 is expressed at lower level than isoform 1 in the other tissues. |
| Function | Receptor activated by multiple ligands, including osteocalcin (BGLAP), basic amino acids, and various cations. Activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Regulates testosterone production by acting as a ligand for uncarboxylated osteocalcin hormone: osteocalcin-binding at the surface of Leydig cells initiates a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Mediates the non-genomic effects of androgens in multiple tissue. May coordinate nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalent ions and its responsiveness to anabolic steroids. |
| Protein Name | G-Protein Coupled Receptor Family C Group 6 Member AHgprc6aG-Protein Coupled Receptor Gpcr33Hgpcr33 |
| Database Links | Reactome: R-HSA-416476Reactome: R-HSA-420499 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-G-Protein Coupled Receptor Family C Group 6 Member A antibodyAnti-Hgprc6a antibodyAnti-G-Protein Coupled Receptor Gpcr33 antibodyAnti-Hgpcr33 antibodyAnti-GPRC6A antibody |
Information sourced from Uniprot.org