![Western blot analysis of HeLa cells, using [KD Validated] glucose-6-phosphateisomerase Rabbit monoclonal antibody (STJ11102070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90987/361690/STJ11102070_2__90207.1713126568.jpg?c=1 "Western blot analysis of HeLa cells, using [KD Validated] glucose-6-phosphateisomerase Rabbit monoclonal antibody (STJ11102070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
Anti-GPI antibody (1-100) [S0MR] (STJ11102070)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal GPI (1-100) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S0MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPI |
| Gene ID | 2821 |
| Uniprot ID | G6PI_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MAALTRDPQFQKLQQWYREH RSELNLRRLFDANKDRFNHF SLTLNTNHGHILVDYSKNLV TEDVMRMLVDLAKSRGVEAA RERMFNGEKINYTEGRAVLH |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Glucose 6 phosphate isomerase (P06744). |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. ISGylated. |
| Function | In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion. |
| Protein Name | Glucose-6-Phosphate IsomeraseGpiAutocrine Motility FactorAmfNeuroleukinNlkPhosphoglucose IsomerasePgiPhosphohexose IsomerasePhiSperm Antigen 36Sa-36 |
| Database Links | Reactome: R-HSA-5628897Reactome: R-HSA-6798695Reactome: R-HSA-70171Reactome: R-HSA-70263 |
| Cellular Localisation | CytoplasmSecreted |
| Alternative Antibody Names | Anti-Glucose-6-Phosphate Isomerase antibodyAnti-Gpi antibodyAnti-Autocrine Motility Factor antibodyAnti-Amf antibodyAnti-Neuroleukin antibodyAnti-Nlk antibodyAnti-Phosphoglucose Isomerase antibodyAnti-Pgi antibodyAnti-Phosphohexose Isomerase antibodyAnti-Phi antibodyAnti-Sperm Antigen 36 antibodyAnti-Sa-36 antibodyAnti-GPI antibody |
Information sourced from Uniprot.org