• Confocal immunofluorescence analysis of Hela cells using GOLPH2 antibody (STJ11103833) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF-7 cells using GOLPH2 antibody (STJ11103833). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GOLPH2 Rabbit polyclonal antibody (STJ11103833) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of Mouse large intestine, using GOLPH2 antibody (STJ11103833) at 1:9000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GOLM1 antibody (112-401) (STJ11103833)

SKU:
STJ11103833

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Golgi Membrane Protein 1 (112-401) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOLM1
Gene ID: 51280
Uniprot ID: GOLM1_HUMAN
Immunogen Region: 112-401
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 112-401 of human GOLPH2 (NP_057632.2).
Immunogen Sequence: TGERLIRVLQDQLKTLQRNY GRLQQDVLQFQKNQTNLERK FSYDLSQCINQMKEVKEQCE ERIEEVTKKGNEAVASRDLS ENNDQRQQLQALSEPQPRLQ AAGLPHTEVPQGKGNVLGNS KSQTPAPSSEVVLDSKRQVE KEETNEIQVVNEEPQRDRLP QEPGREQVVEDRPVGGRGFG GAGELGQTPQVQAALSVSQE NPEMEGPERDQLVIPDGQEE EQEAAGEGRNQQKLRGEDD
Tissue Specificity Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocytes-derived syncytial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.
Post Translational Modifications Glycosylated. Phosphorylation sites are present in the extracellular medium.
Function Unknown. Cellular response protein to viral infection.
Protein Name Golgi Membrane Protein 1
Golgi Membrane Protein Gp73
Golgi Phosphoprotein 2
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Golgi Apparatus
Cis-Golgi Network Membrane
Single-Pass Type Ii Membrane Protein
Early Golgi
Cycles Via The Cell Surface And Endosomes Upon Lumenal Ph Disruption
Alternative Antibody Names Anti-Golgi Membrane Protein 1 antibody
Anti-Golgi Membrane Protein Gp73 antibody
Anti-Golgi Phosphoprotein 2 antibody
Anti-GOLM1 antibody
Anti-C9orf155 antibody
Anti-GOLPH2 antibody
Anti-PSEC0242 antibody
Anti-UNQ686 antibody
Anti-PRO1326 antibody

Information sourced from Uniprot.org

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