• Immunofluorescence analysis of HeLa cells using Giantin Rabbit polyclonal antibody (STJ11104760) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of HeLa, using Giantin antibody (STJ11104760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-GOLGB1 antibody (1123-1302 aa) (STJ11104760)

SKU:
STJ11104760

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOLGB1
Gene ID: 2804
Uniprot ID: GOGB1_HUMAN
Immunogen Region: 1123-1302 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1123-1302 of human Giantin (NP_004478.3).
Immunogen Sequence: QKLITSNTDASDGDSVALVK ETVVISPPCTGSSEHWKPEL EEKILALEKEKEQLQKKLQE ALTSRKAILKKAQEKERHLR EELKQQKDDYNRLQEQFDEQ SKENENIGDQLRQLQIQVRE SIDGKLPSTDQQESCSSTPG LEEPLFKATEQHHTQPVLES NLCPDWPSHSEDASALQGGT
Function May participate in forming intercisternal cross-bridges of the Golgi complex.
Protein Name Golgin Subfamily B Member 1
372 Kda Golgi Complex-Associated Protein
Gcp372
Giantin
Macrogolgin
Database Links Reactome: R-HSA-432722
Reactome: R-HSA-6807878
Reactome: R-HSA-9703465
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Golgin Subfamily B Member 1 antibody
Anti-372 Kda Golgi Complex-Associated Protein antibody
Anti-Gcp372 antibody
Anti-Giantin antibody
Anti-Macrogolgin antibody
Anti-GOLGB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance