Western blot analysis of various lysates using GNG10 Rabbit pAb (STJ110721) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GNG10 antibody (1-68) (STJ110721)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110721
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General Information

Short DescriptionRabbit polyclonal GNG10 (1-68) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolGNG10
Gene ID2790
Uniprot IDGBG10_HUMAN
Immunogen Region1-68
Immunogen SequenceMSSGASASALQRLVEQLKLE AGVERIKVSQAAAELQQYCM QNACKDALLVGVPAGSNPFR EPRSCALL
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human GNG10 (NP_001017998.1).

Additional Info

Tissue Specificity Abundantly and ubiquitously expressed.
Function Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Interacts with beta-1 and beta-2, but not with beta-3.
Protein Name Guanine Nucleotide-Binding Protein G(I/G(S/G(O Subunit Gamma-10
Database Links Reactome: R-HSA-1296041
Reactome: R-HSA-163359
Reactome: R-HSA-202040
Reactome: R-HSA-381676
Reactome: R-HSA-392170
Reactome: R-HSA-392451
Reactome: R-HSA-392851
Reactome: R-HSA-400042
Reactome: R-HSA-4086398
Reactome: R-HSA-416476
Reactome: R-HSA-416482
Reactome: R-HSA-418217
Reactome: R-HSA-418555
Reactome: R-HSA-418592
Reactome: R-HSA-418594
Reactome: R-HSA-418597
Reactome: R-HSA-420092
Reactome: R-HSA-428930
Reactome: R-HSA-432040
Reactome: R-HSA-456926
Reactome: R-HSA-500657
Reactome: R-HSA-6814122
Reactome: R-HSA-8964315
Reactome: R-HSA-8964616
Reactome: R-HSA-9009391
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Reactome: R-HSA-9856530
Reactome: R-HSA-997272
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein G(I/G(S/G(O Subunit Gamma-10 antibody
Anti-GNG10 antibody
Anti-GNGT10 antibody

Information sourced from Uniprot.org

Citations

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