| Host: |
Rabbit |
| Applications: |
WB/IHC-P/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GNE |
| Gene ID: |
10020 |
| Uniprot ID: |
GLCNE_HUMAN |
| Immunogen Region: |
1-150 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GNE (NP_001121699.1). |
| Immunogen Sequence: |
METYGYLQRESCFQGPHELY FKNLSKRNKQIMEKNGNNRK LRVCVATCNRADYSKLAPIM FGIKTEPEFFELDVVVLGSH LIDDYGNTYRMIEQDDFDIN TRLHTIVRGEDEAAMVESVG LALVKLPDVLNRLKPDIMIV HGDRFDALAL |
| Tissue Specificity | Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon. |
| Post Translational Modifications | Phosphorylated. Phosphorylation by PKC activates the UDP-N-acetylglucosamine 2-epimerase activity. |
| Function | Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production of N-acetylneuraminic acid (NeuAc), a critical precursor in the synthesis of sialic acids. By catalyzing this pivotal and rate-limiting step in sialic acid biosynthesis, this enzyme assumes a pivotal role in governing the regulation of cell surface sialylation, playing a role in embryonic angiogenesis. Sialic acids represent a category of negatively charged sugars that reside on the surface of cells as terminal components of glycoconjugates and mediate important functions in various cellular processes, including cell adhesion, signal transduction, and cellular recognition. |
| Protein Name | Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine KinaseUdp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-EpimeraseHydrolyzingUdp-Glcnac-2-EpimeraseUridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine KinaseManac Kinase |
| Database Links | Reactome: R-HSA-4085001Reactome: R-HSA-4085011 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase antibodyAnti-Udp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-Epimerase antibodyAnti-Hydrolyzing antibodyAnti-Udp-Glcnac-2-Epimerase antibodyAnti-Uridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine Kinase antibodyAnti-Manac Kinase antibodyAnti-GNE antibodyAnti-GLCNE antibodyAnti-IBM2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
