• Western blot analysis of extracts of various cell lines, using GNE antibody (STJ111305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using GNE antibody (STJ111305) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using GNE antibody (STJ111305) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using GNE antibody (STJ111305) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophagus using GNE antibody (STJ111305) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using GNE antibody (STJ111305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GNE antibody (1-150) (STJ111305)

SKU:
STJ111305

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GNE (1-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNE
Gene ID: 10020
Uniprot ID: GLCNE_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GNE (NP_001121699.1).
Immunogen Sequence: METYGYLQRESCFQGPHELY FKNLSKRNKQIMEKNGNNRK LRVCVATCNRADYSKLAPIM FGIKTEPEFFELDVVVLGSH LIDDYGNTYRMIEQDDFDIN TRLHTIVRGEDEAAMVESVG LALVKLPDVLNRLKPDIMIV HGDRFDALAL
Tissue Specificity Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon.
Post Translational Modifications Phosphorylated by PKC.
Function Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development. Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.
Protein Name Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase
Udp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-Epimerase
Hydrolyzing
Udp-Glcnac-2-Epimerase
Uridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine Kinase
Manac Kinase
Database Links Reactome: R-HSA-4085001
Reactome: R-HSA-4085011
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase antibody
Anti-Udp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-Epimerase antibody
Anti-Hydrolyzing antibody
Anti-Udp-Glcnac-2-Epimerase antibody
Anti-Uridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine Kinase antibody
Anti-Manac Kinase antibody
Anti-GNE antibody
Anti-GLCNE antibody

Information sourced from Uniprot.org

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