• Western blot analysis of various lysates, using GNAT1 Rabbit polyclonal antibody (STJ11105719) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GNAT1 antibody (58-178) (STJ11105719)

SKU:
STJ11105719

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Guanine Nucleotide-Binding Protein G (T Subunit Alpha-1 (58-178) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNAT1
Gene ID: 2779
Uniprot ID: GNAT1_HUMAN
Immunogen Region: 58-178
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 58-178 of human GNAT1 (NP_000163.2).
Immunogen Sequence: SLEECLEFIAIIYGNTLQSI LAIVRAMTTLNIQYGDSARQ DDARKLMHMADTIEEGTMPK EMSDIIQRLWKDSGIQACFE RASEYQLNDSAGYYLSDLER LVTPGYVPTEQDVLRSRVKT T
Tissue Specificity Rod photoreceptor cells. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium.
Function Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina. Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase.
Protein Name Guanine Nucleotide-Binding Protein G(T Subunit Alpha-1
Transducin Alpha-1 Chain
Database Links Reactome: R-HSA-2485179
Reactome: R-HSA-2514859
Reactome: R-HSA-418594
Cellular Localisation Cell Projection
Cilium
Photoreceptor Outer Segment
Membrane
Peripheral Membrane Protein
Photoreceptor Inner Segment
Localizes Mainly In The Outer Segment In The Dark-Adapted State
Whereas Is Translocated To The Inner Part Of The Photoreceptors In The Light-Adapted State
During Dark-Adapted Conditions
In The Presence Of Unc119 Mislocalizes From The Outer Segment To The Inner Part Of Rod Photoreceptors Which Leads To Decreased Photoreceptor Damage Caused By Light
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein G(T Subunit Alpha-1 antibody
Anti-Transducin Alpha-1 Chain antibody
Anti-GNAT1 antibody
Anti-GNATR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance