| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:100-1:500IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GNAQ |
| Gene ID: |
2776 |
| Uniprot ID: |
GNAQ_HUMAN |
| Immunogen Region: |
1-359 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-359 of human GNAQ (NP_002063.2). |
| Immunogen Sequence: |
MTLESIMACCLSEEAKEARR INDEIERQLRRDKRDARREL KLLLLGTGESGKSTFIKQMR IIHGSGYSDEDKRGFTKLVY QNIFTAMQAMIRAMDTLKIP YKYEHNKAHAQLVREVDVEK VSAFENPYVDAIKSLWNDPG IQECYDRRREYQLSDSTKYY LNDLDRVADPAYLPTQQDVL RVRVPTTGIIEYPFDLQSVI FRMVDVGGQRSERRKWIHCF ENVTSIMFLVALSEYDQVL |
| Tissue Specificity | Predominantly expressed in ovary, prostate, testis and colon. Down-regulated in the peripheral blood lymphocytes (PBLs) of rheumatoid arthritis patients (at protein level). |
| Post Translational Modifications | Palmitoylated by ZDHHC3 and ZDHHC7. Palmitoylation occurs in the Golgi and participates in the localization of GNAQ to the plasma membrane. (Microbial infection) Deamidated at Gln-209 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA. Histaminylated at Gln-209 residues by TGM2. |
| Function | Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation: activates phospholipase C-beta: following GPCR activation, GNAQ activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Required for platelet activation. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs). Together with GNA11, required for heart development. |
| Protein Name | Guanine Nucleotide-Binding Protein G(Q Subunit AlphaGuanine Nucleotide-Binding Protein Alpha-Q |
| Database Links | Reactome: R-HSA-112043Reactome: R-HSA-202040Reactome: R-HSA-399997Reactome: R-HSA-416476Reactome: R-HSA-418592Reactome: R-HSA-428930Reactome: R-HSA-434316Reactome: R-HSA-456926Reactome: R-HSA-6814122 |
| Cellular Localisation | Cell MembraneLipid-AnchorGolgi ApparatusNucleusNucleus MembraneColocalizes With The Adrenergic ReceptorsAdren1a And Adren1bAt The Nuclear Membrane Of Cardiac Myocytes |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein G(Q Subunit Alpha antibodyAnti-Guanine Nucleotide-Binding Protein Alpha-Q antibodyAnti-GNAQ antibodyAnti-GAQ antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
