• Immunohistochemistry analysis of paraffin-embedded mouse testis using Glycerol kinase (GK) Rabbit monoclonal antibody (STJ11102954) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Glycerol kinase (GK) Rabbit monoclonal antibody (STJ11102954) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Glycerol kinase (GK) (GK) antibody (STJ11102954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using Glycerol kinase (GK) (GK) antibody (STJ11102954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Glycerol kinase antibody (450-559) [S4MR] (STJ11102954)

SKU:
STJ11102954

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GK
Gene ID: 2710
Uniprot ID: GLPK_HUMAN
Immunogen Region: 450-559
Specificity: A synthetic peptide corresponding to a sequence within amino acids 450-559 of human Glycerol kinase (GK) (P32189).
Immunogen Sequence: IPVVKPSMPETTALGAAMAA GAAEGVGVWSLEPEDLSAVT MERFEPQINAEESEIRYSTW KKAVMKSMGWVTTQSPESGD PSIFCSLPLGFFIVSSMVML IGARYISGIP
Tissue Specificity Isoform 2: Widely expressed in fetal and adult tissues. Isoform 3: Widely expressed in fetal and adult tissues. Isoform 4: The sole isoform expressed in adult liver and kidney.
Function Kinase that plays a key role in glycerol metabolism, catalyzing its phosphorylation to produce sn-glycerol 3-phosphate. Sn-glycerol 3-phosphate is a crucial intermediate in various metabolic pathways, such as the synthesis of glycerolipids and triglycerides, glycogenesis, glycolysis and gluconeogenesis.
Protein Name Glycerol Kinase
Glycerokinase
Atp -Glycerol 3-Phosphotransferase
Database Links Reactome: R-HSA-75109
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Nucleus
Cytoplasm
Cytosol
Glycerol Kinase Activity Is More Cytosolic In Some Tissues
It Probably Represents The Expression Of Isoforms Lacking A Transmembrane Domain
Isoform 3: Mitochondrion Outer Membrane
In Sperm And Fetal Tissues
The Majority Of The Activity Is Associated With Mitochondria
Isoform 4: Cytoplasm
In Adult Tissues
Such As Liver The Glycerol Kinase Activity Is More Cytosolic
It Probably Represents The Expression Of This Isoform Which Lacks A Transmembrane Domain
Alternative Antibody Names Anti-Glycerol Kinase antibody
Anti-Glycerokinase antibody
Anti-Atp -Glycerol 3-Phosphotransferase antibody
Anti-GK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance