• Western blot analysis of extracts of various cell lines, using GLUD1 rabbit monoclonal antibody (STJ11102284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using GLUD1 rabbit monoclonal antibody (STJ11102284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded human colon carcinoma tissue using GLUD1 rabbit monoclonal antibody (STJ11102284) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded human colon tissue using GLUD1 rabbit monoclonal antibody (STJ11102284) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded human liver cancer tissue using GLUD1 rabbit monoclonal antibody (STJ11102284) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded human thyroid cancer tissue using GLUD1 rabbit monoclonal antibody (STJ11102284) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded mouse brain tissue using GLUD1 rabbit monoclonal antibody (STJ11102284) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded mouse kidney tissue using GLUD1 rabbit monoclonal antibody (STJ11102284) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded rat colon tissue using GLUD1 rabbit monoclonal antibody (STJ11102284) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded rat liver tissue using GLUD1 rabbit monoclonal antibody (STJ11102284) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of NIH-3T3 cells using GLUD1 rabbit monoclonal antibody (STJ11102284) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GLUD1 antibody (459-558) [S4MR] (STJ11102284)

SKU:
STJ11102284

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GLUD1 (459-558) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLUD1
Gene ID: 2746
Uniprot ID: DHE3_HUMAN
Immunogen Region: 459-558
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 459-558 of human GLUD1 (P00367).
Immunogen Sequence: ERDSNYHLLMSVQESLERKF GKHGGTIPIVPTAEFQDRIS GASEKDIVHSGLAYTMERSA RQIMRTAMKYNLGLDLRTAA YVNAIEKVFKVYNEAGVTFT
Post Translational Modifications ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity. Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.
Function Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
Protein Name Glutamate Dehydrogenase 1 - Mitochondrial
Gdh 1
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-8964539
Cellular Localisation Mitochondrion
Endoplasmic Reticulum
Mostly Translocates Into The Mitochondria
Only A Small Amount Of The Protein Localizes To The Endoplasmic Reticulum
Alternative Antibody Names Anti-Glutamate Dehydrogenase 1 - Mitochondrial antibody
Anti-Gdh 1 antibody
Anti-GLUD1 antibody
Anti-GLUD antibody

Information sourced from Uniprot.org

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