Anti-GLS antibody (610-669) (STJ23808)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GLS (610-669) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GLS |
| Gene ID | 2744 |
| Uniprot ID | GLSK_HUMAN |
| Immunogen Region | 610-669 |
| Immunogen Sequence | KVNPFPKDRWNNTPMDEALH FGHHDVFKILQEYQVQYTPQ GDSDNGKENQTVHKNLDGLL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human Glutaminase (Glutaminase (GLS) ) (NP_055720.3). |
Additional Info
| Tissue Specificity | Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle. |
| Post Translational Modifications | Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits. |
| Function | Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain. Isoform 2: Lacks catalytic activity. |
| Protein Name | Glutaminase Kidney Isoform - MitochondrialGlsK-GlutaminaseL-Glutamine Amidohydrolase Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain |
| Database Links | Reactome: R-HSA-210500Reactome: R-HSA-5628897Reactome: R-HSA-8964539 |
| Cellular Localisation | Isoform 1: MitochondrionCytoplasmCytosolThe 74-Kda Cytosolic Precursor Is Translocated Into The Mitochondria And Processed Via A 72-Kda Intermediate To Yield The Mature 68- And 65-Kda SubunitsIsoform 3: MitochondrionGlutaminase Kidney IsoformMitochondrial 68 Kda Chain: Mitochondrion MatrixProduced By The Proteolytic Processing Of The 74-Kda Cytosolic PrecursorMitochondrial 65 Kda Chain: Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Glutaminase Kidney Isoform - Mitochondrial antibodyAnti-Gls antibodyAnti-K-Glutaminase antibodyAnti-L-Glutamine Amidohydrolase Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain antibodyAnti-GLS antibodyAnti-GLS1 antibodyAnti-KIAA0838 antibody |
Information sourced from Uniprot.org