• Western blot analysis of various lysates, using GLIS3 Rabbit polyclonal antibody (STJ114059) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GLIS3 antibody (606-775) (STJ114059)

SKU:
STJ114059

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLIS3 (606-775) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLIS3
Gene ID: 169792
Uniprot ID: GLIS3_HUMAN
Immunogen Region: 606-775
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 606-775 of human GLIS3 (NP_689842.3).
Immunogen Sequence: DAGAERFAPSAPSPHHISPR RVPAPSSILQRTQPPYTQQP SGSHLKSYQPETNSSFQPNG IHVHGFYGQLQKFCPPHYPD SQRIVPPVSSCSVVPSFEDC LVPTSMGQASFDVFHRAFST HSGITVYDLPSSSSSLFGES LRSGAEDATFLQISTVDRCP SQLSSVYTEG
Tissue Specificity In the adult, expressed at high levels in the kidney and at lower levels in the brain, skeletal muscle, pancreas, liver, lung, thymus and ovary.
Function Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'.
Protein Name Zinc Finger Protein Glis3
Gli-Similar 3
Zinc Finger Protein 515
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger Protein Glis3 antibody
Anti-Gli-Similar 3 antibody
Anti-Zinc Finger Protein 515 antibody
Anti-GLIS3 antibody
Anti-ZNF515 antibody

Information sourced from Uniprot.org

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