Anti-GK antibody (422-559) (STJ11100926)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GK (422-559) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GK |
| Gene ID | 2710 |
| Uniprot ID | GLPK_HUMAN |
| Immunogen Region | 422-559 |
| Immunogen Sequence | GIPLSHLQVDGGMTSNKILM QLQADILYIPVVKPSMPETT ALGAAMAAGAAEGVGVWSLE PEDLSAVTMERFEPQINAEE SEIRYSTWKKAVMKSMGWVT TQSPESGDPSIFCSLPLGFF IVSSMVMLIGARYISGIP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 422-559 of Glycerol kinase (GK) (NP_001191948.1). |
Additional Info
| Tissue Specificity | Isoform 2: Widely expressed in fetal and adult tissues. Isoform 3: Widely expressed in fetal and adult tissues. Isoform 4: The sole isoform expressed in adult liver and kidney. |
| Function | Kinase that plays a key role in glycerol metabolism, catalyzing its phosphorylation to produce sn-glycerol 3-phosphate. Sn-glycerol 3-phosphate is a crucial intermediate in various metabolic pathways, such as the synthesis of glycerolipids and triglycerides, glycogenesis, glycolysis and gluconeogenesis. |
| Protein Name | Glycerol KinaseGlycerokinaseAtp -Glycerol 3-Phosphotransferase |
| Database Links | Reactome: R-HSA-75109 |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Membrane ProteinNucleusCytoplasmCytosolGlycerol Kinase Activity Is More Cytosolic In Some TissuesIt Probably Represents The Expression Of Isoforms Lacking A Transmembrane DomainIsoform 3: Mitochondrion Outer MembraneIn Sperm And Fetal TissuesThe Majority Of The Activity Is Associated With MitochondriaIsoform 4: CytoplasmIn Adult TissuesSuch As Liver The Glycerol Kinase Activity Is More CytosolicIt Probably Represents The Expression Of This Isoform Which Lacks A Transmembrane Domain |
| Alternative Antibody Names | Anti-Glycerol Kinase antibodyAnti-Glycerokinase antibodyAnti-Atp -Glycerol 3-Phosphotransferase antibodyAnti-GK antibody |
Information sourced from Uniprot.org