Anti-GGT1 antibody (Internal) (STJ72965)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-GGT1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 60kDa band observed in Human Kidney lysates (calculated MW of 61.4kDa according to NP_005256.2). Recommended concentration: 0.3-1ยตg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | GGT1 |
| Gene ID | 2678 |
| Uniprot ID | GGT1_HUMAN |
| Accession Number | NP_005256.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | PSASKEPDNHVYTR |
| Specificity | Reported variants represent identical protein: NP_038347.2, NP_001027536.1, NP_001027537.1, NP_005256.2. |
Additional Info
| Post Translational Modifications | N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity. Cleaved by autocatalysis into a large and a small subunit and the autocatalytic cleavage is essential to the functional activation of the enzyme. |
| Function | Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4. Isoform 3: Seems to be inactive. |
| Protein Name | Glutathione Hydrolase 1 ProenzymeGamma-Glutamyltransferase 1Gamma-Glutamyltranspeptidase 1Ggt 1Leukotriene-C4 HydrolaseCd Antigen Cd224 Cleaved Into - Glutathione Hydrolase 1 Heavy Chain - Glutathione Hydrolase 1 Light Chain |
| Database Links | Reactome: R-HSA-174403Reactome: R-HSA-2142691Reactome: R-HSA-5423646Reactome: R-HSA-5579022Reactome: R-HSA-9035968Reactome: R-HSA-9664535Reactome: R-HSA-9753281 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Glutathione Hydrolase 1 Proenzyme antibodyAnti-Gamma-Glutamyltransferase 1 antibodyAnti-Gamma-Glutamyltranspeptidase 1 antibodyAnti-Ggt 1 antibodyAnti-Leukotriene-C4 Hydrolase antibodyAnti-Cd Antigen Cd224 Cleaved Into - Glutathione Hydrolase 1 Heavy Chain - Glutathione Hydrolase 1 Light Chain antibodyAnti-GGT1 antibodyAnti-GGT antibody |
Information sourced from Uniprot.org