Anti-GFP antibody (STJ140006)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant peptide produced in E. coli.
General Information
| Short Description | Goat polyclonal Green Fluorescent Protein antibody for use in WB, IF, IHC-P and IHC-F in gfp samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/IHC-P/IHC-F |
| Host | Goat |
| Reactivity | GFP |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 3 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:500-1:5000IF 1:50-1:1000IHC-P 1:50-1:1000IHC-F 1:50-1:1000Serifi I Besta S Karetsou Z et al. Sci Rep 2021 Jul PMID: 34230583Almeida F Luís MP Pereira IS et al. Front Cell Infect Microbiol 2018 Jul PMID: 30094225Issa |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in-20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence | MVSKGEELFTGVVPILVELD VSKGEELFTGVVPILVELDG SKGEELFTGVVPILVELDGD KGEELFTGVVPILVELDGDV GEELFTGVVPILVELDGDVN EELFTGVVPILVELDGDVNG ELFTGVVPILVELDGDVNGH LFTGVVPILVELDGDVNGHK FTGVVPILVELDGDVNGHKF TGVVPILVELDGDVNGHKFS GVVPILVELDGDVNGHKFSV VVPILVELDGDVNGHKFSV |
| Specificity | In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein. |
Additional Info
| Background | Goat polyclonal antibody to GFP (green fluorescent protein). |
Information sourced from Uniprot.org