| Host: | Goat |
| Applications: | WB/IF/IHC-P/IHC-F |
| Reactivity: | GFP |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal anti-Green Fluorescent Protein for use in WB, IF, IHC-P and IHC-F in GFP samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 3 mg/mL |
| Dilution Range: | WB 1:500-1:5000IF 1:50-1:1000IHC-P 1:50-1:1000IHC-F 1:50-1:1000Serifi I Besta S Karetsou Z et al. Sci Rep 2021 Jul PMID: 34230583Almeida F Luís MP Pereira IS et al. Front Cell Infect Microbiol 2018 Jul PMID: 30094225Issa |
| Storage Instruction: | For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in-20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Specificity: | In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein. |
| Immunogen: | Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence: | MVSKGEELFTGVVPILVELD VSKGEELFTGVVPILVELDG SKGEELFTGVVPILVELDGD KGEELFTGVVPILVELDGDV GEELFTGVVPILVELDGDVN EELFTGVVPILVELDGDVNG ELFTGVVPILVELDGDVNGH LFTGVVPILVELDGDVNGHK FTGVVPILVELDGDVNGHKF TGVVPILVELDGDVNGHKFS GVVPILVELDGDVNGHKFSV VVPILVELDGDVNGHKFSV |
| Background | Goat polyclonal antibody to GFP (green fluorescent protein). |
Information sourced from Uniprot.org
