• Anti-GFP antibody at 1:2000 dilution; transfected 293HEK cell lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Hepa1-6 transfected with GFP-Rab1a and immunostained with with GFP antibody

Anti-GFP antibody (STJ140006)

SKU:
STJ140006

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF
Reactivity: GFP
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Green Fluorescent Protein is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:5000
IF 1:50-1:1000
IHC-P 1:50-1:1000
IHC-F 1:50-1:1000
Serifi I Besta S Karetsou Z et al. Sci Rep 2021 Jul PMID: 34230583
Almeida F Luís MP Pereira IS et al. Front Cell Infect Microbiol 2018 Jul PMID: 30094225
Issa
Storage Instruction: For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in-20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.
Immunogen: Purified recombinant peptide produced in E. coli.
Immunogen Sequence: MVSKGEELFTGVVPILVELD GDVNGHKFSVSGEGEGDATY GKLTLKFICTTGKLPVPWPT LVTTLTYGVQCFSRYPDHMK QHDFFKSAMPEGYVQERTIF FKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGH KLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLA DHYQQNTPIGDGPVLLPDNH YLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK
Background Goat polyclonal antibody to GFP (green fluorescent protein).

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance