| Host: | Goat |
| Applications: | WB/IF/IHC-P/IHC-F |
| Reactivity: | GFP |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal anti-Green Fluorescent Protein for use in WB, IF, IHC-P and IHC-F in GFP samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 2 mg/mL |
| Dilution Range: | WB 1:500-1:5000IF 1:50-1:1000IHC-P 1:50-1:1000IHC-F 1:50-1:1000Cabaço LC Bento-Lopes L Neto MV et al. JID Innov 2022 Jun PMID: 36090299Vyas P Wu JS Zimmerman A et al. J Assoc Res Otolaryngol 2016 Sep PMID: 27696081Larson |
| Storage Instruction: | Store at-20 C for long-term storage. Store at 2-8 C for up to one month. |
| Specificity: | In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins. |
| Immunogen: | Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence: | MVSKGEELFTGVVPILVELD VSKGEELFTGVVPILVELDG SKGEELFTGVVPILVELDGD KGEELFTGVVPILVELDGDV GEELFTGVVPILVELDGDVN EELFTGVVPILVELDGDVNG ELFTGVVPILVELDGDVNGH LFTGVVPILVELDGDVNGHK FTGVVPILVELDGDVNGHKF TGVVPILVELDGDVNGHKFS GVVPILVELDGDVNGHKFSV VVPILVELDGDVNGHKFSV |
| Background | Goat polyclonal antibody to GFP (green fluorescent protein). |
Information sourced from Uniprot.org
