| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GFI1B (50-150) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GFI1B |
| Gene ID: |
8328 |
| Uniprot ID: |
GFI1B_HUMAN |
| Immunogen Region: |
50-150 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GFI1B (NP_001128503.1). |
| Immunogen Sequence: |
FPNQCLDWTNLKREPELEQD QNLARMAPAPEGPIVLSRPQ DGDSPLSDSPPFYKPSFSWD TLATTYGHSYRQAPSTMQSA FLEHSVSLYGSPLVPSTEPA L |
| Tissue Specificity | Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA). |
| Post Translational Modifications | Methylation at Lys-8 in the SNAG domain seems required for the recruitment of the corepressor complex. |
| Function | Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context.represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1.GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription.as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. |
| Protein Name | Zinc Finger Protein Gfi-1bGrowth Factor Independent Protein 1bPotential Regulator Of Cdkn1a Translocated In Cml |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Zinc Finger Protein Gfi-1b antibodyAnti-Growth Factor Independent Protein 1b antibodyAnti-Potential Regulator Of Cdkn1a Translocated In Cml antibodyAnti-GFI1B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
