• Immunohistochemistry analysis of GFAP. Sample: mouse brain, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:600, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of GFAP. Sample: Rat brain, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:600, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • IF analysis of GFAP. Sample: mouse brain, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • IF analysis of GFAP. Sample: Rat brain, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • IF analysis of GFAP. Sample: Rat brain, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.

Anti-GFAP antibody [SM0405] (STJA0010405)

SKU:
STJA0010405

£170.50 - £233.50
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Host: Mouse
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-GFAP is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM0405
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (M, R) 1:500-1:5000
IHC/IF (H, M, R) 1:500-1:2000/1:500-1:2000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Gfap
Gene ID: 14580
Uniprot ID: GFAP_MOUSE
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse GFAP
Post Translational Modifications Phosphorylated by PKN1.
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Protein Name Glial Fibrillary Acidic Protein
Gfap
Cellular Localisation Cytoplasm
Associated With Intermediate Filaments
Alternative Antibody Names Anti-Glial Fibrillary Acidic Protein antibody
Anti-Gfap antibody
Anti-Gfap antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance