• Fluorescence multiplex immunohistochemical analysis of mouse brain tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Sextuple-Fluorescence kit. GFAP mouse monoclonal antibody (STJA0006242 green), S100 mouse monoclonal antibody (STJ197266 cyan), Neurofilament mouse monoclonal antibody (purple), Iba 1 mouse monoclonal antibody (red), CD8 a mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).
  • mouse brain tissue was stained with Anti-GFAP (PTR2201/470) Antibody
  • mouse brain tissue was stained with Anti-GFAP (PTR2201/470) Antibody

Anti-GFAP antibody (300-432) [PTR2201/470] (STJA0006242)

SKU:
STJA0006242

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Glial fibrillary acidic protein (300-432) is suitable for use in Immunohistochemistry, Western Blot and ELISA research applications.
Clonality: Monoclonal
Clone ID: PTR2201/470
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was purified using affinity-chromatography using specific immunogen.
Dilution Range: IHC-p 1:200-400
WB 1:200-1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GFAP
Gene ID: 2670
Uniprot ID: GFAP_HUMAN
Immunogen Region: 300-432
Specificity: The antibody can specifically recognize human GFAP protein.
Immunogen: Synthesized peptide derived from human GFAP AA range: 300-432
Tissue Specificity Expressed in cells lacking fibronectin.
Post Translational Modifications Phosphorylated by PKN1.
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Protein Name Glial Fibrillary Acidic Protein
Gfap
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-9613829
Cellular Localisation Cytoplasm
Associated With Intermediate Filaments
Alternative Antibody Names Anti-Glial Fibrillary Acidic Protein antibody
Anti-Gfap antibody
Anti-GFAP antibody

Information sourced from Uniprot.org

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