• Western blot analysis of mouse brain, using GFAP rabbit polyclonal antibody (STJ23770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using GFAP rabbit polyclonal antibody (STJ23770) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human brain using GFAP rabbit polyclonal antibody (STJ23770) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using GFAP rabbit polyclonal antibody (STJ23770) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat brain cells using GFAP rabbit polyclonal antibody (STJ23770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain cells using GFAP rabbit polyclonal antibody (STJ23770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-251MG cells using GFAP rabbit polyclonal antibody (STJ23770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain cells using GFAP rabbit polyclonal antibody (STJ23770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain cells using GFAP rabbit polyclonal antibody (STJ23770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GFAP antibody (1-75) (STJ23770)

SKU:
STJ23770

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GFAP (1-75) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GFAP
Gene ID: 2670
Uniprot ID: GFAP_HUMAN
Immunogen Region: 1-75
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-75 of human GFAP (NP_002046.1).
Immunogen Sequence: MERRRITSAARRSYVSSGEM MVGGLAPGRRLGPGTRLSLA RMPPPLPTRVDFSLAGALNA GFKETRASERAEMME
Tissue Specificity Expressed in cells lacking fibronectin.
Post Translational Modifications Phosphorylated by PKN1.
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Protein Name Glial Fibrillary Acidic Protein
Gfap
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-9613829
Cellular Localisation Cytoplasm
Associated With Intermediate Filaments
Alternative Antibody Names Anti-Glial Fibrillary Acidic Protein antibody
Anti-Gfap antibody
Anti-GFAP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance