• Western blot analysis of lysate from COLO205 cells, using GDF-9 antibody.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of GDF-9 antibody in paraffin-embedded human liver carcinoma tissue.
  • Western blot analysis of various cells using GDF-9 Polyclonal Antibody

Anti-GDF9 antibody (273-322 aa) (STJ93256)

SKU:
STJ93256

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Growth/differentiation factor 9 (273-322 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GDF9
Gene ID: 2661
Uniprot ID: GDF9_HUMAN
Immunogen Region: 273-322 aa
Specificity: GDF-9 Polyclonal Antibody detects endogenous levels of GDF-9 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GDF-9 at the amino acid range 273-322
Post Translational Modifications Phosphorylated.phosphorylation is critical for GDF9 function. In vitro, can be phosphorylated by CK at Ser-325.
Function Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Protein Name Growth/Differentiation Factor 9
Gdf-9
Cellular Localisation Secreted
Alternative Antibody Names Anti-Growth/Differentiation Factor 9 antibody
Anti-Gdf-9 antibody
Anti-GDF9 antibody

Information sourced from Uniprot.org

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