• Immunohistochemistry analysis of paraffin-embedded rat brain using GATA2 antibody (STJ23753) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using GATA2 antibody (STJ23753) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of HeLa, using GATA2 antibody (STJ23753) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GATA2 antibody (1-300) (STJ23753)

SKU:
STJ23753

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GATA2 (1-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA2
Gene ID: 2624
Uniprot ID: GATA2_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GATA2 (NP_116027.2).
Immunogen Sequence: MEVAPEQPRWMAHPAVLNAQ HPDSHHPGLAHNYMEPAQLL PPDEVDVFFNHLDSQGNPYY ANPAHARARVSYSPAHARLT GGQMCRPHLLHSPGLPWLDG GKAALSAAAAHHHNPWTVSP FSKTPLHPSAAGGPGGPLSV YPGAGGGSGGGSGSSVASLT PTAAHSGSHLFGFPPTPPKE VSPDPSTTGAASPASSSAGG SAARGEDKDGVKYQVSLTES MKMESGSPLRPGLATMGTQ
Tissue Specificity Endothelial cells.
Function Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
Protein Name Endothelial Transcription Factor Gata-2
Gata-Binding Protein 2
Database Links Reactome: R-HSA-8939236
Reactome: R-HSA-9616222
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Endothelial Transcription Factor Gata-2 antibody
Anti-Gata-Binding Protein 2 antibody
Anti-GATA2 antibody

Information sourced from Uniprot.org

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