| Host: | Goat |
| Applications: | WB/IHC-F/IF |
| Reactivity: | Human/Rat/Mouse/Zebrafish/Canine/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-GAPDH (240 aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | DyLight®405 |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol, 0.05% ProClin® and 0.05% Sodium Azide. |
| Purification: | Epitope affinity purified |
| Concentration: | 2 mg/mL |
| Dilution Range: | WB 1:500-1:5000 IHC-F 1:200-1:1000 IF 1:50-1:250 |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: | GAPDH |
| Gene ID: | 2597 |
| Uniprot ID: | G3P_HUMAN |
| Immunogen Region: | 240 aa C-Term |
| Specificity: | Detects a band of 37 kDa by Western blot. |
| Immunogen: | Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli. |
| Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. |
| Protein Name | Glyceraldehyde-3-Phosphate Dehydrogenase Gapdh Peptidyl-Cysteine S-Nitrosylase Gapdh |
| Database Links | Reactome: R-HSA-70171 Reactome: R-HSA-70263 |
| Cellular Localisation | Cytoplasm Cytosol Nucleus Perinuclear Region Membrane Cytoskeleton Translocates To The Nucleus Following S-Nitrosylation And Interaction With Siah1 Which Contains A Nuclear Localization Signal Postnuclear And Perinuclear Regions |
| Alternative Antibody Names | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase antibody Anti-Gapdh antibody Anti-Peptidyl-Cysteine S-Nitrosylase Gapdh antibody Anti-GAPDH antibody Anti-GAPD antibody Anti-CDABP0047 antibody Anti-OK antibody Anti-SW-cl.12 antibody |
Information sourced from Uniprot.org