Anti-Gankyrin/PSMD10 antibody (1-226aa) [AT1F4] (STJA0041761)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenRecombinant human PSMD10 (1-226aa) purified from E. coli
General Information
| Short Description | Mouse monoclonal anti-Gankyrin/PSMD10 (1-226aa) for use in ELISA, WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC/IF |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | AT1F4 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | By protein-A affinity chromatography |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PSMD10 |
| Gene ID | 5716 |
| Uniprot ID | PSD10_HUMAN |
| Accession Number | NP_002805 |
| Immunogen | Recombinant human PSMD10 (1-226aa) purified from E. coli |
| Immunogen Region | 1-226aa |
Additional Info
| Tissue Specificity | Tends to be up-regulated in cancer cells with RAS mutations, including lung cancers and adenocarconimas (at protein level). |
| Function | Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export. |
| Protein Name | 26s Proteasome Non-Atpase Regulatory Subunit 1026s Proteasome Regulatory Subunit P28GankyrinP28(Gank |
| Database Links | Reactome: R-HSA-9907900 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-26s Proteasome Non-Atpase Regulatory Subunit 10 antibodyAnti-26s Proteasome Regulatory Subunit P28 antibodyAnti-Gankyrin antibodyAnti-P28(Gank antibodyAnti-PSMD10 antibody |
Information sourced from Uniprot.org