• Immunofluorescence analysis of U-2 OS cells using GALNT3 Rabbit polyclonal antibody (STJ115920) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GALNT3 Rabbit polyclonal antibody (STJ115920) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GALNT3 antibody (STJ115920) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GALNT3 antibody (43-633) (STJ115920)

SKU:
STJ115920

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GALNT3 (43-633) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GALNT3
Gene ID: 2591
Uniprot ID: GALT3_HUMAN
Immunogen Region: 43-633
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 43-633 of human GALNT3 (NP_004473.2).
Immunogen Sequence: VQYSKEESRMERNMKNKNKM LDLMLEAVNNIKDAMPKMQI GAPVRQNIDAGERPCLQGYY TAAELKPVLDRPPQDSNAPG ASGKAFKTTNLSVEEQKEKE RGEAKHCFNAFASDRISL
Tissue Specificity Expressed in organs that contain secretory epithelial glands. Highly expressed in pancreas, skin, kidney and testis. Weakly expressed in prostate, ovary, intestine and colon. Also expressed in placenta and lung and fetal lung and fetal kidney.
Function Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward HIV envelope glycoprotein gp120, EA2, MUC2, MUC1A and MUC5AC. Probably glycosylates fibronectin in vivo. Glycosylates FGF23.
Protein Name Polypeptide N-Acetylgalactosaminyltransferase 3
Polypeptide Galnac Transferase 3
Galnac-T3
Pp-Gantase 3
Protein-Udp Acetylgalactosaminyltransferase 3
Udp-Galnac -Polypeptide N-Acetylgalactosaminyltransferase 3
Database Links Reactome: R-HSA-190372
Reactome: R-HSA-5083625
Reactome: R-HSA-913709
Cellular Localisation Golgi Apparatus
Golgi Stack Membrane
Single-Pass Type Ii Membrane Protein
Resides Preferentially In The Trans And Medial Parts Of The Golgi Stack
Alternative Antibody Names Anti-Polypeptide N-Acetylgalactosaminyltransferase 3 antibody
Anti-Polypeptide Galnac Transferase 3 antibody
Anti-Galnac-T3 antibody
Anti-Pp-Gantase 3 antibody
Anti-Protein-Udp Acetylgalactosaminyltransferase 3 antibody
Anti-Udp-Galnac -Polypeptide N-Acetylgalactosaminyltransferase 3 antibody
Anti-GALNT3 antibody

Information sourced from Uniprot.org

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