• Immunofluorescence analysis of mouse eye cells using GAD67/GAD1 Rabbit polyclonal antibody (STJ23736) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat eye cells using GAD67/GAD1 Rabbit polyclonal antibody (STJ23736) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using GAD67/GAD67/GAD1 Rabbit polyclonal antibody (STJ23736) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of 293T cells, using (STJ23736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of Mouse lung, using (STJ23736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GAD1 antibody (1-200) (STJ23736)

SKU:
STJ23736

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GAD1 (1-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GAD1
Gene ID: 2571
Uniprot ID: DCE1_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GAD67/GAD67/GAD1 (NP_000808.2).
Immunogen Sequence: MASSTPSSSATSSNAGADPN TTNLRPTTYDTWCGVAHGCT RKLGLKICGFLQRTNSLEEK SRLVSAFKERQSSKNLLSCE NSDRDARFRRTETDFSNLFA RDLLPAKNGEEQTVQFLLEV VDILLNYVRKTFDRSTKVLD FHHPHQLLEGMEGFNLELSD HPESLEQILVDCRDTLKYGV RTGHPRFFNQLSTGLDIIGL
Tissue Specificity Isoform 1: Expressed in brain. Isoform 3: Expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
Function Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor. Isoform 3: Enzymatically inactive as glutamate decarboxylase.
Protein Name Glutamate Decarboxylase 1
67 Kda Glutamic Acid Decarboxylase
Gad-67
Glutamate Decarboxylase 67 Kda Isoform
Database Links Reactome: R-HSA-888568
Reactome: R-HSA-888590
Reactome: R-HSA-9022927
Alternative Antibody Names Anti-Glutamate Decarboxylase 1 antibody
Anti-67 Kda Glutamic Acid Decarboxylase antibody
Anti-Gad-67 antibody
Anti-Glutamate Decarboxylase 67 Kda Isoform antibody
Anti-GAD1 antibody
Anti-GAD antibody
Anti-GAD67 antibody

Information sourced from Uniprot.org

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