| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Gamma-aminobutyric acid receptor-associated protein-like 2 receptor-associated protein-like 2 (26-75 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:10000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GABARAPL2 |
| Gene ID: | 11345 |
| Uniprot ID: | GBRL2_HUMAN |
| Immunogen Region: | 26-75 aa |
| Specificity: | GABARAP Polyclonal Antibody detects endogenous levels of GABARAP |
| Immunogen: | Synthetic peptide of GABARAP at the amino acid range 26-75 |
| Post Translational Modifications | The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, GABARAPL2-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, GABARAPL2-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms required for GABARAPL2 recycling when autophagosomes fuse with lysosomes. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation at Ser-87 and Ser-88 by TBK1 prevents interaction with ATG4 (ATG4A, ATG4B, ATG4C or ATG4D). Phosphorylation by TBK1 on autophagosomes prevents their delipidation by ATG4 and premature removal from nascent autophagosomes. |
| Function | Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. |
| Protein Name | Gamma-Aminobutyric Acid Receptor-Associated Protein-Like 2 Gaba(A Receptor-Associated Protein-Like 2 Ganglioside Expression Factor 2 Gef-2 General Protein Transport Factor P16 Golgi-Associated Atpase Enhancer Of 16 Kda Gate-16 Map1 Light Chain 3-Related Protein |
| Database Links | Reactome: R-HSA-1632852 Reactome: R-HSA-8854214 |
| Cellular Localisation | Cytoplasmic Vesicle Autophagosome Endoplasmic Reticulum Membrane Golgi Apparatus |
| Alternative Antibody Names | Anti-Gamma-Aminobutyric Acid Receptor-Associated Protein-Like 2 antibody Anti-Gaba(A Receptor-Associated Protein-Like 2 antibody Anti-Ganglioside Expression Factor 2 antibody Anti-Gef-2 antibody Anti-General Protein Transport Factor P16 antibody Anti-Golgi-Associated Atpase Enhancer Of 16 Kda antibody Anti-Gate-16 antibody Anti-Map1 Light Chain 3-Related Protein antibody Anti-GABARAPL2 antibody Anti-FLC3A antibody Anti-GEF2 antibody |
Information sourced from Uniprot.org