| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-G6PD (305-318) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 55kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 59.3kDa according to NP_001035810.1). Recommended concentration: 1-3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | G6PD |
| Gene ID: | 2539 |
| Uniprot ID: | G6PD_HUMAN |
| Immunogen Region: | 305-318 |
| Accession Number: | NP_000393.4 ; NP_001035810.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1). |
| Immunogen Sequence: | KPASTNSDDVRDEK |
| Post Translational Modifications | Acetylated by ELP3 at Lys-403.acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403.deacetylation stimulates its enzyme activity. |
| Function | Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. |
| Protein Name | Glucose-6-Phosphate 1-DehydrogenaseG6pd |
| Database Links | Reactome: R-HSA-5628897Reactome: R-HSA-71336Reactome: R-HSA-9818028 |
| Cellular Localisation | CytoplasmCytosolMembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Glucose-6-Phosphate 1-Dehydrogenase antibodyAnti-G6pd antibodyAnti-G6PD antibody |
Information sourced from Uniprot.org
