Anti-FZD8/frizzled 8 antibody (C-Term) (STJ70823)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-FZD8/frizzled 8 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and EM research applications. |
| Applications | Pep-ELISA/WB/IHC/IF/EM |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75kDa band observed in Human Pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3ยตg/ml. Primary incubation was 1 hour. This antib |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | FZD8 |
| Gene ID | 8325 |
| Uniprot ID | FZD8_HUMAN |
| Accession Number | NP_114072.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | SYPKQMPLSQV |
Additional Info
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. |
| Protein Name | Frizzled-8Fz-8Hfz8 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-4608870Reactome: R-HSA-4641263Reactome: R-HSA-5340588 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinGolgi ApparatusCell MembraneColocalizes With Gopc At The Golgi Apparatus |
| Alternative Antibody Names | Anti-Frizzled-8 antibodyAnti-Fz-8 antibodyAnti-Hfz8 antibodyAnti-FZD8 antibody |
Information sourced from Uniprot.org